| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Pseudomonas fragi | TGGATCGCCTGCTTTGCA | CTCGAACTTGCCCAGCGAT | 59.97 | 60.45 | 159 | 128 |
100.00%
|
100.00%
|
| Primer 2 | Pseudomonas fragi | TCCTCGAGTTGTTGCGCA | AATTGGTCGGCTTGGGCA | 59.58 | 59.88 | 186 | 128 |
100.00%
|
100.00%
|
| Primer 3 | Pseudomonas fragi | CGCATCCATCCCATCGAGT | AATTGAGCAGGCCACGCA | 59.63 | 60.28 | 265 | 128 |
100.00%
|
100.00%
|
| Primer 4 | Pseudomonas fragi | ACGCATCCATCCCATCGAG | AATTGAGCAGGCCACGCA | 59.63 | 60.28 | 266 | 128 |
100.00%
|
100.00%
|
| Primer 5 | Pseudomonas fragi | ACCAACGCCTTTGCTCCA | GCTCGATCACAGCTTGCAG | 59.81 | 59.29 | 157 | 125 |
100.00%
|
100.00%
|
| Primer 6 | Pseudomonas fragi | TGCTTGAAGCCCACGCAT | ACATAGCCCTGCATCACCG | 60.28 | 59.85 | 244 | 125 |
100.00%
|
100.00%
|
| Primer 7 | Pseudomonas fragi | TGGATCGCCTGCTTTGCA | TGCTGCATGGTGGAGTGA | 59.97 | 58.84 | 200 | 122 |
100.00%
|
100.00%
|
| Primer 8 | Pseudomonas fragi | TGGATCGCCTGCTTTGCA | AACTTGCCCAGCGATACGG | 59.97 | 60.45 | 155 | 120 |
100.00%
|
100.00%
|
| Primer 9 | Pseudomonas fragi | ATTGCTGTTGCCGTGTGC | AACGAACGCAACACCAGC | 59.66 | 59.29 | 206 | 118 |
100.00%
|
100.00%
|
| Primer 10 | Pseudomonas fragi | TGAAGGCAAGACCGTGAGC | TGCCCACCGAGCTTTTGT | 60.30 | 59.81 | 176 | 118 |
100.00%
|
100.00%
|